01.
NMNHinowedzera maseru NAD + uye NADH mazinga. Vatsvagiri vakafananidza mhedzisiro yeNMN neNMNH pakuwedzera maseru NAD + mazinga. Kurapa neNMNH (100 μM) kwakawedzera maseru NAD+ mazinga ne5-fold muHepG2 maseru, nepo 100 μM NMN yakawedzera zvishoma NAD+ mazinga. Pamusoro peHepG2 masero, vaongorori vakasimbisawo kuti NMNH yakasimudzira NAD + muES-2 maseru uye 3T3-L1 masero, ari emunhu maovary-akabva masero uye mbeva embryonic fibroblast-yakatorwa maseru, zvichiteerana. Uye vaongorori vakawanawo kuti NMNH yakawedzera maseru NAD + mazinga mu786-O masero munguva- uye inotsamira-maitiro. Vatsvakurudzi vakayera NADH vachishandisa mass spectrometry uye vakawana kuti mazinga eNADH muNMNH-akarapwa HepG2 masero akanga ari 2.5 nguva dzakakwirira kudarika masero asina kurapwa uye NMN-akabatwa, uye mazinga akakwirira eNADH akaonekwawo muNMNH-yakarapwa 786-O masero. Takaonawo kuti 340 mg/kg NMNH kurapwa yakawedzera NADH mazinga mumbeva zviropa ne3-fold, nepo 340 mg/kg NMN kurapwa yakawedzera NADH mazinga ne1.7-fold.
Mufananidzo 1.2. NMNH inowedzera NAD + mazinga
Mufananidzo 3.4. NMNH inowedzera NMNH mazinga
02NMNH inhibits glycolysis uye TCA kutenderera. Iko kuunganidzwa kweNADH kunoderedza glycolysis uye TCA kutenderera. Kuti uongorore mhinduro yemetabolism yemasero kuNMNH kurapwa, vaongorori vakaita metabolomic ongororo ye
NMNH-uye masero anorapwa neNMN. Yakawanikwa kuti kurapwa kweNMNH hakuna kungoderedza mazinga eglycolysis intermediates, asiwo yakaderedza mazinga eTCA cycle intermediates. Izvi zvinoratidza kuti kuunganidzwa kweNADH inogadzirwa neNMNH inhibits cellular glycolysis uye TCA cycle.
Mufananidzo 5. Yakaderedzwa glycolysis uye TCA cycle intermediates
Mufananidzo 6. Metabolomics kuongorora
03.
NMNHinhibits cell growth Vatsvakurudzi vakaongorora mhedzisiro yeNMNH pakukura kwesero uye vakawana kuti kurapwa kweNMNH kwakadzivisa HepG2 kukura kwesero pamatunhu akakwirira kupfuura 250 μM. Vatsvakurudzi vakaongorora kutenderera kwesero muHepG2 masero akabatwa ne1 mM NMNH kana NMN kwemaawa 12 uye akawana kuti NMNH yakakonzera kusungwa kwesero, nepo NMN yakanga isina simba pane sero cycle. Mukuwedzera, vatsvakurudzi vakawanawo kuti 500 μM NMNH yakavhara zvachose kukura kwe786-O masero; 250 μM NMNH inogona kunyatso kuvharidzira kukura kwesero muHK-2 masero.
Mufananidzo 7.8.NMNH inhibits cell growth in vitro
04.
NMNHvatsvakurudzi vekuchengetedza vakaita zviedzo pamusoro pekuti madosi makuru eNMNH anokonzera hepatotoxicity. Kurapa kweC57BL/6J mbeva dzechirume dzine 50, 100, 500, kana 1000 mg/kg NMNH kuburikidza nejekiseni remukati mezuva rimwe nerimwe kwevhiki imwe kwakaratidza kusaderera kweserum alanine aminotransferase (ALT) uye aspartate aminotransferase (AST) mazinga. Yakasimudzwa, zvichiratidza kuti NMNH haisi hepatotoxic pamadosi makuru. Pamusoro pezvo, vaongorori vakawanawo kuti kurapwa kweNMNH kwakawedzera iyo NAD + zvirimo mumakonzo zviropa nenzira inoenderana nedosi. Kurapa ne1000 mg/kg NMNH kwakawedzera mazinga eNAD+ muzviropa zvembeva nekanopfuura ka10.
Mufananidzo 10.
NMNHinowedzera mwero weNAD + muchiropa chembeva
Zvidzidzo zvakaratidza kuti NMNH iri nani NAD+ -boosting supplement pane NMN zvese mu vitro uye in vivo; nekuwedzera, NMNH inowedzera yakaderedzwa NAD (NADH) mazinga mumasero uye mbeva zviropa. Metabolomic analysis yakaratidza kuti NMNH inhibits glycolysis uye TCA cycle. In vitro zviedzo zvinoratidza kuti NMNH induces cell cycle kusungwa uye inhibits kukura kwesero. Zvakatorwa pamwechete, chidzidzo ichi chinoratidza kuti NMNH ine simba NAD + inosimudzira uye inodzivirira glycolysis, TCA kutenderera, uye sero kukura.
Zita rechiChinese reNMNH "rakaderedzwa β-nicotinamide mononucleotide", iyo ndiyo yakaderedzwa yeNMN uye inonzi amorphous yellow powder. Molecular formula: C11H17N2O8P Molecular uremu: 336.23g/molCAS Nha.: 108347-85-9 Mabhuku ekutsvagisa achangoburwa anoratidza kuti NMNH inowedzera NAD + mazinga nekukurumidza uye paavhareji, paavhareji 5 nguva iyo yeNMN
HealthwayNMNHpfuti chaiyo
